sp1120  (Vector Laboratories)

Journal: iScience

Article Title: The first interneuron of the mouse visual system is tailored to the natural environment through morphology and electrical coupling

doi: 10.1016/j.isci.2024.111276

Figure Lengend Snippet: Spatial localization of horizontal cell gap junctions (A–C) Projections of a confocal image stack of a vertical cryostat section double-labeled for the kainate receptor subunit 1 (GluK1, used as a cone pedicle marker) and the horizontal cell gap junction protein connexin 57 (Cx57). (D–F) Maximum intensity projections of a confocal image stack of whole-mounted retina labeled with antibodies against Cx57 and with Alexa Fluor 568-conjugated streptavidin after microinjection of neurobiotin into a horizontal cell. The distribution of Cx57 is shown at the tips of horizontal cell (HC) dendrites invaginating into two cone pedicles. Pedicle positions were identified by these clusters of invaginating dendritic tips and are indicated by dashed circles. (G–I) As in (D) and (F), but ∼2 μm beneath the same pedicle position. INL, inner nuclear layer; OPL, outer plexiform layer. Scale bars: 10 μm in (C), applies to (A)–(C); 5 μm in (I), applies to (D)–(I).

Article Snippet: Electrodes were tip-filled with 3 μL of a 1:1 mixture of 4% neurobiotin (SP1120, Biozol) diluted in 0.1 M Tris buffer (pH 7.3) and 5 mM Alexa Fluor 568 Hydrazide, and back-filled with 10 μL of 200 mM KCl in Tris buffer.

Techniques: Labeling, Marker, Microinjection

Journal: iScience

Article Title: The first interneuron of the mouse visual system is tailored to the natural environment through morphology and electrical coupling

doi: 10.1016/j.isci.2024.111276

Figure Lengend Snippet: Tracer coupling patterns of horizontal cells differ in dorsal and ventral retina (A) The position of neurobiotin-injected horizontal cells is indicated by circles superimposed on the density distribution disc taken from Figure 1 E. The area of the circles reflects the number of tracer-coupled cell bodies per injected horizontal cell. Orange and blue filled circles represent the example injections in (B)–(E). (B and C) Example tracer-coupling patterns upon injection of neurobiotin into single cell bodies (B, in dorsal retina; C, ventral). Convex hulls encompassing all neurobiotin-positive cell bodies were used to measure the area of neurobiotin-spread. (D and E) Quantification of the number of tracer-coupled horizontal cell bodies (D, p < 0.01) and the area of tracer-spread (E, p < 0.001) per injection (Mann-Whitney tests). Vertical lines indicate median. Scale bar 100 μm in (C), applies to (B) and (C).

Article Snippet: Electrodes were tip-filled with 3 μL of a 1:1 mixture of 4% neurobiotin (SP1120, Biozol) diluted in 0.1 M Tris buffer (pH 7.3) and 5 mM Alexa Fluor 568 Hydrazide, and back-filled with 10 μL of 200 mM KCl in Tris buffer.

Techniques: Injection, MANN-WHITNEY

Journal: iScience

Article Title: The first interneuron of the mouse visual system is tailored to the natural environment through morphology and electrical coupling

doi: 10.1016/j.isci.2024.111276

Figure Lengend Snippet:

Article Snippet: Electrodes were tip-filled with 3 μL of a 1:1 mixture of 4% neurobiotin (SP1120, Biozol) diluted in 0.1 M Tris buffer (pH 7.3) and 5 mM Alexa Fluor 568 Hydrazide, and back-filled with 10 μL of 200 mM KCl in Tris buffer.

Techniques: Recombinant, Software